Sodium boric acid: a Tris-free, cooler conductive medium for DNA electrophoresis
نویسندگان
چکیده
منابع مشابه
Sodium boric acid: a Tris-free, cooler conductive medium for DNA electrophoresis.
BENCHMARKS It has been nearly three decades since the invention of DNA electro-phoresis (1−6). Current conductive media for DNA electrophoresis remain largely restricted to legacy Tris-acetic acid-disodium EDTA (TAE) and Tris-boric acid-disodium EDTA (TBE) at substantial ionic strengths, leading to higher cost and excessive heat generation , and limiting the voltage and speed of electrophoretic...
متن کاملIntroducing a New and Simple Protocol for Capillary Electrophoresis of Cell Free Fetal Double Stranded DNA
Isolation of cell free fetal DNA (cffDNA) from maternal serum usually leads to very low concentrations of DNA impeding further resolving through conventional methods of electrophoresis. Although several protocols have been described for capillary electrophoresis (CE) of double stranded DNA, they usually need using special polymers or coated capillaries which degrade over time. Herein, we propos...
متن کاملHistory and principles of conductive media for standard DNA electrophoresis.
DNA electrophoresis has been a dominant technique in molecular biology for 30 years. The foundation for this common technique is based on a few simple electrochemical principles. Electrophoretic DNA separation borrowed from existing protein and RNA techniques developed in the 1950s and 1960s. For 30 years, common DNA electrophoretic conductive media remained largely unchanged, with Tris as the ...
متن کاملintroducing a new and simple protocol for capillary electrophoresis of cell free fetal double stranded dna
isolation of cell free fetal dna (cffdna) from maternal serum usually leads to very low concentrations of dna impeding further resolving through conventional methods of electrophoresis. although several protocols have been described for capillary electrophoresis (ce) of double stranded dna, they usually need using special polymers or coated capillaries which degrade over time. herein, we propos...
متن کاملBoric acid lactose broth as a medium for the detection of fecal coliform bacteria.
Boric acid lactose broth has been suggested (Levine et al., 1934; Vaughn and Levine, 1935; Vaughn et al., 1951; Levine et al., 1955) as a medium for the detection of Escherichia coli in water and in foods. This medium is designed to suppress Escherichia freundii and species of Aerobacter, organisms often considered to be nonfecal in origin. The published results are rather convincing where sepa...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2004
ISSN: 0736-6205,1940-9818
DOI: 10.2144/04362bm02